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Human acid sphingomyelinase structures provide insight to molecular basis of Niemann-Pick disease.


ABSTRACT: Acid sphingomyelinase (ASM) hydrolyzes sphingomyelin to ceramide and phosphocholine, essential components of myelin in neurons. Genetic alterations in ASM lead to ASM deficiency (ASMD) and have been linked to Niemann-Pick disease types A and B. Olipudase alfa, a recombinant form of human ASM, is being developed as enzyme replacement therapy to treat the non-neurological manifestations of ASMD. Here we present the human ASM holoenzyme and product bound structures encompassing all of the functional domains. The catalytic domain has a metallophosphatase fold, and two zinc ions and one reaction product phosphocholine are identified in a histidine-rich active site. The structures reveal the underlying catalytic mechanism, in which two zinc ions activate a water molecule for nucleophilic attack of the phosphodiester bond. Docking of sphingomyelin provides a model that allows insight into the selectivity of the enzyme and how the ASM domains collaborate to complete hydrolysis. Mapping of known mutations provides a basic understanding on correlations between enzyme dysfunction and phenotypes observed in ASMD patients.

SUBMITTER: Zhou YF 

PROVIDER: S-EPMC5062611 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Human acid sphingomyelinase structures provide insight to molecular basis of Niemann-Pick disease.

Zhou Yan-Feng YF   Metcalf Matthew C MC   Garman Scott C SC   Edmunds Tim T   Qiu Huawei H   Wei Ronnie R RR  

Nature communications 20161011


Acid sphingomyelinase (ASM) hydrolyzes sphingomyelin to ceramide and phosphocholine, essential components of myelin in neurons. Genetic alterations in ASM lead to ASM deficiency (ASMD) and have been linked to Niemann-Pick disease types A and B. Olipudase alfa, a recombinant form of human ASM, is being developed as enzyme replacement therapy to treat the non-neurological manifestations of ASMD. Here we present the human ASM holoenzyme and product bound structures encompassing all of the functiona  ...[more]

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2018-12-28 | MSV000083287 | MassIVE