Dataset Information
Core/shell protein-reactive nanogels via a combination of RAFT polymerization and vinyl sulfone postmodification.
Ontology highlight
ABSTRACT: Aim
A promising nanogel vaccine platform was expanded toward antigen conjugation.Materials & methods
Block copolymers containing a reactive ester solvophobic block and a PEG-like solvophilic block were synthesized via reversible addition-fragmentation chain-transfer polymerization. Following self-assembly in DMSO, the esters allow for core-crosslinking and hydrophilization by amide bond formation with primary amines. Free thiols were accessed at the polymer chain ends through aminolysis of the reversible addition-fragmentation chain-transfer groups, and into the nanogel core by reactive ester conversion with cysteamine. Subsequently, free thiols were converted into vinyl sulfone moieties.Results
Despite sterical constraints, nanogel-associated vinyl sulfone moieties remained well accessible for cysteins to enforce protein conjugation successfully.Conclusion
Our present findings provide a next step toward well-defined vaccine nanoparticles that can co-deliver antigen and a molecular adjuvant.
SUBMITTER: Vanparijs N
PROVIDER: S-EPMC5066120 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Publications
Core/shell protein-reactive nanogels via a combination of RAFT polymerization and vinyl sulfone postmodification.
Nanomedicine (London, England) 20160915 20
<h4>Aim</h4>A promising nanogel vaccine platform was expanded toward antigen conjugation.<h4>Materials & methods</h4>Block copolymers containing a reactive ester solvophobic block and a PEG-like solvophilic block were synthesized via reversible addition-fragmentation chain-transfer polymerization. Following self-assembly in DMSO, the esters allow for core-crosslinking and hydrophilization by amide bond formation with primary amines. Free thiols were accessed at the polymer chain ends through ami ...[more]