Core/shell protein-reactive nanogels via a combination of RAFT polymerization and vinyl sulfone postmodification.

ABSTRACT: A promising nanogel vaccine platform was expanded toward antigen conjugation.Block copolymers containing a reactive ester solvophobic block and a PEG-like solvophilic block were synthesized via reversible addition-fragmentation chain-transfer polymerization. Following self-assembly in DMSO, the esters allow for core-crosslinking and hydrophilization by amide bond formation with primary amines. Free thiols were accessed at the polymer chain ends through aminolysis of the reversible addition-fragmentation chain-transfer groups, and into the nanogel core by reactive ester conversion with cysteamine. Subsequently, free thiols were converted into vinyl sulfone moieties.Despite sterical constraints, nanogel-associated vinyl sulfone moieties remained well accessible for cysteins to enforce protein conjugation successfully.Our present findings provide a next step toward well-defined vaccine nanoparticles that can co-deliver antigen and a molecular adjuvant.

SUBMITTER: Vanparijs N 

PROVIDER: S-EPMC5066120 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature