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Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus.

ABSTRACT: Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligonucleotide ligation assay (OLA) for HBV lamivudine-resistance was developed and compared to consensus sequencing. Both assays detected drug resistance mutations in 35/64 (54.7%) specimens evaluated, and OLA detected minority mutants in an additional six (9.4%). OLA may offer a sensitive and inexpensive alternative to consensus sequencing for detection of HBV drug resistance in resource-limited settings.

SUBMITTER: Beck IA

PROVIDER: S-EPMC5074383 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Development and validation of an oligonucleotide ligation assay to detect lamivudine resistance in hepatitis B virus.

Beck Ingrid A IA Payant Rachel R Ngo-Giang-Huong Nicole N Khamduang Woottichai W Laomanit Laddawan L Jourdain Gonzague G Frenkel Lisa M LM

Journal of virological methods 20160326

Treatment of chronic hepatitis B virus (HBV) infection with lamivudine-monotherapy rapidly selects mutant variants in a high proportion of individuals. Monitoring lamivudine resistance by consensus sequencing is costly and insensitive for detection of minority variants. An oligonucleotide ligation assay (OLA) for HBV lamivudine-resistance was developed and compared to consensus sequencing. Both assays detected drug resistance mutations in 35/64 (54.7%) specimens evaluated, and OLA detected minor ...[more]

PMID: 27025356

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