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Optical and nuclear imaging of glioblastoma with phosphatidylserine-targeted nanovesicles.


ABSTRACT: Multimodal tumor imaging with targeted nanoparticles potentially offers both enhanced specificity and sensitivity, leading to more precise cancer diagnosis and monitoring. We describe the synthesis and characterization of phenol-substituted, lipophilic orange and far-red fluorescent dyes and a simple radioiodination procedure to generate a dual (optical and nuclear) imaging probe. MALDI-ToF analyses revealed high iodination efficiency of the lipophilic reporters, achieved by electrophilic aromatic substitution using the chloramide 1,3,4,6-tetrachloro-3?,6?-diphenyl glycoluril (Iodogen) as the oxidizing agent in an organic/aqueous co-solvent mixture. Upon conjugation of iodine-127 or iodine-124-labeled reporters to tumor-targeting SapC-DOPS nanovesicles, optical (fluorescent) and PET imaging was performed in mice bearing intracranial glioblastomas. In addition, tumor vs non-tumor (normal brain) uptake was compared using iodine-125. These data provide proof-of-principle for the potential value of SapC-DOPS for multimodal imaging of glioblastoma, the most aggressive primary brain tumor.

SUBMITTER: Blanco VM 

PROVIDER: S-EPMC5078058 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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Optical and nuclear imaging of glioblastoma with phosphatidylserine-targeted nanovesicles.

Blanco Víctor M VM   Chu Zhengtao Z   LaSance Kathleen K   Gray Brian D BD   Pak Koon Yan KY   Rider Therese T   Greis Kenneth D KD   Qi Xiaoyang X  

Oncotarget 20160501 22


Multimodal tumor imaging with targeted nanoparticles potentially offers both enhanced specificity and sensitivity, leading to more precise cancer diagnosis and monitoring. We describe the synthesis and characterization of phenol-substituted, lipophilic orange and far-red fluorescent dyes and a simple radioiodination procedure to generate a dual (optical and nuclear) imaging probe. MALDI-ToF analyses revealed high iodination efficiency of the lipophilic reporters, achieved by electrophilic aromat  ...[more]

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