Intact Cell MALDI-TOF MS on Sperm: A Molecular Test For Male Fertility Diagnosis.

ABSTRACT: Currently, evaluation of sperm quality is primarily based on in vitro measures of sperm function such as motility, viability and/or acrosome reaction. However, results are often poorly correlated with fertility, and alternative diagnostic tools are therefore needed both in veterinary and human medicine. In a recent pilot study, we demonstrated that MS profiles from intact chicken sperm using MALDI-TOF profiles could detect significant differences between fertile/subfertile spermatozoa showing that such profiles could be useful for in vitro male fertility testing. In the present study, we performed larger standardized experimental procedures designed for the development of fertility- predictive mathematical models based on sperm cell MALDI-TOF MS profiles acquired through a fast, automated

SUBMITTER: Soler L 

PROVIDER: S-EPMC5083112 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature