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DNA Origami Seesaws as Comparative Binding Assay.

ABSTRACT: The application of commonly used force spectroscopy in biological systems is often limited by the need for an invasive tether connecting the molecules of interest to a bead or cantilever tip. Here we present a DNA origami-based prototype in a comparative binding assay. It has the advantage of in situ readout without any physical connection to the macroscopic world. The seesaw-like structure has a lever that is able to move freely relative to its base. Binding partners on each side force the structure into discrete and distinguishable conformations. Model experiments with competing DNA hybridisation reactions yielded a drastic shift towards the conformation with the stronger binding interaction. With reference DNA duplexes of tuneable length on one side, this device can be used to measure ligand interactions in comparative assays.

SUBMITTER: Nickels PC

PROVIDER: S-EPMC5084756 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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DNA Origami Seesaws as Comparative Binding Assay.

Nickels Philipp C PC Høiberg Hans C HC Simmel Stephanie S SS Holzmeister Phil P Tinnefeld Philip P Liedl Tim T

Chembiochem : a European journal of chemical biology 20160506 12

The application of commonly used force spectroscopy in biological systems is often limited by the need for an invasive tether connecting the molecules of interest to a bead or cantilever tip. Here we present a DNA origami-based prototype in a comparative binding assay. It has the advantage of in situ readout without any physical connection to the macroscopic world. The seesaw-like structure has a lever that is able to move freely relative to its base. Binding partners on each side force the stru ...[more]

PMID: 27038073

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