Superstructure-Dependent Loading of DNA Origami Nanostructures with a Groove-Binding Drug.

ABSTRACT: DNA origami nanostructures are regarded as powerful and versatile vehicles for targeted drug delivery. So far, DNA origami-based drug delivery strategies mostly use intercalation of the therapeutic molecules between the base pairs of the DNA origami's double helices for drug loading. The binding of nonintercalating drugs to DNA origami nanostructures, however, is less studied. Therefore, in this work, we investigate the interaction of the drug methylene blue (MB) with different DNA origami nanostructures under conditions that result in minor groove binding. We observe a noticeable effect of DNA origami superstructure on the binding affinity of MB. In particular, non-B topologies as for instance found in designs using the square lattice with 10.67 bp/turn may result in reduced binding affin

SUBMITTER: Kollmann F 

PROVIDER: S-EPMC6644410 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature