Unknown

Dataset Information

0

Desmin and ?B-crystallin interplay in the maintenance of mitochondrial homeostasis and cardiomyocyte survival.


ABSTRACT: The association of desmin with the ?-crystallin ?-chain (??-crystallin; encoded by CRYAB), and the fact that mutations in either one of them leads to heart failure in humans and mice, suggests a potential compensatory interplay between the two in cardioprotection. To address this hypothesis, we investigated the consequences of ??-crystallin overexpression in the desmin-deficient (Des-/-) mouse model, which possesses a combination of the pathologies found in most cardiomyopathies, with mitochondrial defects as a hallmark. We demonstrated that cardiac-specific ??-crystallin overexpression ameliorates all these defects and improves cardiac function to almost wild-type levels. Protection by ??-crystallin overexpression is linked to maintenance of proper mitochondrial protein levels, inhibition of abnormal mitochondrial permeability transition pore activation and maintenance of mitochondrial membrane potential (??m). Furthermore, we found that both desmin and ??-crystallin are localized at sarcoplasmic reticulum (SR)-mitochondria-associated membranes (MAMs), where they interact with VDAC, Mic60 - the core component of mitochondrial contact site and cristae organizing system (MICOS) complex - and ATP synthase, suggesting that these associations could be crucial in mitoprotection at different levels.

SUBMITTER: Diokmetzidou A 

PROVIDER: S-EPMC5087667 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

altmetric image

Publications

Desmin and αB-crystallin interplay in the maintenance of mitochondrial homeostasis and cardiomyocyte survival.

Diokmetzidou Antigoni A   Soumaka Elisavet E   Kloukina Ismini I   Tsikitis Mary M   Makridakis Manousos M   Varela Aimilia A   Davos Constantinos H CH   Georgopoulos Spiros S   Anesti Vasiliki V   Vlahou Antonia A   Capetanaki Yassemi Y  

Journal of cell science 20160826 20


The association of desmin with the α-crystallin Β-chain (αΒ-crystallin; encoded by CRYAB), and the fact that mutations in either one of them leads to heart failure in humans and mice, suggests a potential compensatory interplay between the two in cardioprotection. To address this hypothesis, we investigated the consequences of αΒ-crystallin overexpression in the desmin-deficient (Des<sup>-/-</sup>) mouse model, which possesses a combination of the pathologies found in most cardiomyopathies, with  ...[more]

Similar Datasets

| S-EPMC1851605 | biostudies-literature
| S-EPMC1398051 | biostudies-literature
| S-EPMC4992032 | biostudies-literature
| S-EPMC3834786 | biostudies-literature
| S-EPMC2773995 | biostudies-literature
| S-EPMC3602778 | biostudies-literature
| S-EPMC9633452 | biostudies-literature
| S-EPMC3212511 | biostudies-literature
| S-EPMC4860071 | biostudies-literature
| S-EPMC6405666 | biostudies-literature