Dataset Information

The multifaceted nature of ?B-crystallin.

ABSTRACT: In vivo, small heat-shock proteins (sHsps) are key players in maintaining a healthy proteome. ?B-crystallin (?B-c) or HspB5 is one of the most widespread and populous of the ten human sHsps. Intracellularly, ?B-c acts via its molecular chaperone action as the first line of defence in preventing target protein unfolding and aggregation under conditions of cellular stress. In this review, we explore how the structure of ?B-c confers its function and interactions within its oligomeric self, with other sHsps, and with aggregation-prone target proteins. Firstly, the interaction between the two highly conserved regions of ?B-c, the central ?-crystallin domain and the C-terminal IXI motif and how this regulates ?B-c chaperone activity are explored. Secondly, subunit exchange is rationalised as an integral structural and functional feature of ?B-c. Thirdly, it is argued that monomeric ?B-c may be its most chaperone-species active, but at the cost of increased hydrophobicity and instability. Fourthly, the reasons why hetero-oligomerisation of ?B-c with other sHsps is important in regulating cellular proteostasis are examined. Finally, the interaction of ?B-c with aggregation-prone, partially folded target proteins is discussed. Overall, this paper highlights the remarkably diverse capabilities of ?B-c as a caretaker of the cell.

SUBMITTER: Hayashi J

PROVIDER: S-EPMC7332646 | biostudies-literature | 2020 Jul

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

The multifaceted nature of αB-crystallin.

Hayashi Junna J Carver John A JA

Cell stress & chaperones 20200507 4

In vivo, small heat-shock proteins (sHsps) are key players in maintaining a healthy proteome. αB-crystallin (αB-c) or HspB5 is one of the most widespread and populous of the ten human sHsps. Intracellularly, αB-c acts via its molecular chaperone action as the first line of defence in preventing target protein unfolding and aggregation under conditions of cellular stress. In this review, we explore how the structure of αB-c confers its function and interactions within its oligomeric self, with ot ...[more]

PMID: 32383140

Similar Datasets

Project description:In his seminal book on esthetics, Berlyne (1971) posited an inverted-U relationship between complexity and hedonic tone in arts appreciation, however, converging evidence for his theory is still missing. The disregard of the multidimensionality of complexity may explain some of the divergent results. Here, we argue that definitions of hedonic tone are manifold and systematically examined whether the nature of the relationship between complexity and hedonic tone is determined by the specific measure of hedonic tone. In Experiment 1, we studied three picture categories with similar affective and semantic contents: 96 affective environmental scenes, which were also converted into 96 cartoons, and 96 representational paintings. Complexity varied along the dimension of elements. In a between-subjects design, each stimulus was presented for 5 s to 206 female participants. Subjective ratings of hedonic tone (either beauty, pleasantness or liking), arousal, complexity and familiarity were collected in three conditions per stimulus set. Complexity and arousal were positively associated in all conditions, with the strongest association observed for paintings. For environmental scenes and cartoons, there was no significant association between complexity and hedonic tone, and the three measures of hedonic tone were highly correlated (all rs > 0.85). As predicted, in paintings the measures of hedonic tone were less strongly correlated (all rs > 0.73), and when controlling for familiarity, the association with complexity was significantly positive for beauty (rs = 0.26), weakly negative for pleasantness (rs = -0.16) and not present for liking. Experiment 2 followed a similar approach and 77 female participants, all non-musicians, rated 92 musical excerpts (15 s) in three conditions of hedonic tone (either beauty, pleasantness or liking). Results indicated a strong relationship between complexity and arousal (all rs > 0.85). When controlling for familiarity effects, the relationship between complexity and beauty followed an inverted-U curve, whereas the relationship between complexity and pleasantness was negative (rs = -0.26) and the one between complexity and liking positive (rs = 0.29). We relate our results to Berlyne's theory and the latest findings in neuroaesthetics, proposing that future studies need to acknowledge the multifaceted nature of hedonic tone in esthetic experiences of artforms.

Dataset Information

The multifaceted nature of ?B-crystallin.

Publications

The multifaceted nature of αB-crystallin.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets