Dataset Information

Integrated Electrodes and Electrospray Emitter for Polymer Microfluidic Nanospray-MS Interface.

ABSTRACT: Interfacing of microfluidic devices to mass spectrometry has challenges including dilution from sheath liquid junctions, fragile electrodes, and excessive dead volumes which prevent optimum performance and common use. The goal of this work is to develop a stable nanospray chip-MS interface that contains easily integrated electrodes and an embedded capillary emitter to mitigate current chip-MS problems. This system uses a hybrid polystyrene-poly(dimethylsiloxane) (PS-PDMS) microfluidic platform with an embedded electrode and integrated capillary emitter used as the nanospray interface. Two chip designs were used to evaluate the performance, illustrate on-chip reaction capabilities. By direct infusion, this system showed good performance with LODs of GSH and caffeine of 9 nM and 1 nM, R² of 0.996 and 0.992 and sensitivity of 12 counts/nM and 332 counts/nM over a linear dynamic range of 40 nM to 50 ?M and 1 to 50 ?M respectively. A reaction was performed on the chip with syringe pumps showing the oxidation of glutathione (GSH) to oxidized glutathione (GSSG) using H₂O₂. The on-chip reaction of GSH oxidation to GSSG, with online-MS detection, successfully demonstrate the stability and robustness of the nanospray interface.

SUBMITTER: Forzano AV

PROVIDER: S-EPMC5091296 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

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Integrated Electrodes and Electrospray Emitter for Polymer Microfluidic Nanospray-MS Interface.

Forzano Anna V AV Becirovic Vedada V Martin R Scott RS Edwards James L JL

Analytical methods : advancing methods and applications 20160601 25

Interfacing of microfluidic devices to mass spectrometry has challenges including dilution from sheath liquid junctions, fragile electrodes, and excessive dead volumes which prevent optimum performance and common use. The goal of this work is to develop a stable nanospray chip-MS interface that contains easily integrated electrodes and an embedded capillary emitter to mitigate current chip-MS problems. This system uses a hybrid polystyrene-poly(dimethylsiloxane) (PS-PDMS) microfluidic platform w ...[more]

PMID: 27818712

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Project description:Lipids are a naturally occurring group of molecules that not only contribute to the structural integrity of the lung preventing alveolar collapse but also play important roles in the anti-inflammatory responses and antiviral protection. Alteration in the type and spatial localization of lipids in the lung plays a crucial role in various diseases, such as respiratory distress syndrome (RDS) in preterm infants and oxidative stress-influenced diseases, such as pneumonia, emphysema, and lung cancer following exposure to environmental stressors. The ability to accurately measure spatial distributions of lipids and metabolites in lung tissues provides important molecular insights related to lung function, development, and disease states. Nanospray desorption electrospray ionization (nano-DESI) and other ambient ionization mass spectrometry techniques enable label-free imaging of complex samples in their native state with minimal to absolutely no sample preparation. However, lipid coverage obtained in nano-DESI mass spectrometry imaging (MSI) experiments has not been previously characterized. In this work, the depth of lipid coverage in nano-DESI MSI of mouse lung tissues was compared to liquid chromatography tandem mass spectrometry (LC-MS/MS) lipidomics analysis of tissue extracts prepared using two different procedures: standard Folch extraction method of the whole lung samples and extraction into a 90% methanol/10% water mixture used in nano-DESI MSI experiments. A combination of positive and negative ionization mode nano-DESI MSI identified 265 unique lipids across 20 lipids subclasses and 19 metabolites (284 in total) in mouse lung tissues. Except for triacylglycerols (TG) species, nano-DESI MSI provided comparable coverage to LC-MS/MS experiments performed using methanol/water tissue extracts and up to 50% coverage in comparison with the Folch extraction-based whole lung lipidomics analysis. These results demonstrate the utility of nano-DESI MSI for comprehensive spatially resolved analysis of lipids in tissue sections. A combination of nano-DESI MSI and LC-MS/MS lipidomics is particularly useful for exploring changes in lipid distributions during lung development, as well as resulting from disease or exposure to environmental toxicants.

Dataset Information

Integrated Electrodes and Electrospray Emitter for Polymer Microfluidic Nanospray-MS Interface.

Publications

Integrated Electrodes and Electrospray Emitter for Polymer Microfluidic Nanospray-MS Interface.

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