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Metabolic effects of basic fibroblast growth factor in streptozotocin-induced diabetic rats: A ¹H NMR-based metabolomics investigation.

ABSTRACT: The fibroblast growth factors (FGFs) family shows a great potential in the treatment of diabetes, but little attention is paid to basic FGF (bFGF). In this study, to explore the metabolic effects of bFGF on diabetes, metabolic changes in serum and feces were analyzed in the normal rats, the streptozocin (STZ)-induced diabetic rats and the bFGF-treated diabetic rats using a ¹H nuclear magnetic resonance (NMR)-based metabolomic approach. Interestingly, bFGF treatment significantly decreased glucose, lipid and low density lipoprotein/very low density lipoprotein (LDL/VLDL) levels in serum of diabetic rats. Moreover, bFGF treatment corrected diabetes-induced reductions in citrate, lactate, choline, glycine, creatine, histidine, phenylalanine, tyrosine and glutamine in serum. Fecal propionate was significantly increased after bFGF treatment. Correlation analysis shows that glucose, lipid and LDL/VLDL were significantly negatively correlated with energy metabolites (citrate, creatine and lactate) and amino acids (alanine, glycine, histidine, phenylalanine, tyrosine and glutamine). In addition, a weak but significant correlation was observed between fecal propionate and serum lipid (R?=?-0.35, P?=?0.046). Based on metabolic correlation and pathway analysis, therefore, we suggest that the glucose and lipid lowering effects of bFGF in the STZ-induced diabetic rats may be achieved by activating microbial metabolism, increasing energy metabolism and correcting amino acid metabolism.

SUBMITTER: Lin X

PROVIDER: S-EPMC5093531 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Metabolic effects of basic fibroblast growth factor in streptozotocin-induced diabetic rats: A <sup>1</sup>H NMR-based metabolomics investigation.

Lin Xiaodong X Zhao Liangcai L Tang Shengli S Zhou Qi Q Lin Qiuting Q Li Xiaokun X Zheng Hong H Gao Hongchang H

Scientific reports 20161103

The fibroblast growth factors (FGFs) family shows a great potential in the treatment of diabetes, but little attention is paid to basic FGF (bFGF). In this study, to explore the metabolic effects of bFGF on diabetes, metabolic changes in serum and feces were analyzed in the normal rats, the streptozocin (STZ)-induced diabetic rats and the bFGF-treated diabetic rats using a <sup>1</sup>H nuclear magnetic resonance (NMR)-based metabolomic approach. Interestingly, bFGF treatment significantly decre ...[more]

PMID: 27808173

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Project description:IntroductionErythromycin (ERY) is known to induce hepatic toxicity which mimics other liver diseases. Thus, ERY is often used to produce experimental models of drug-induced liver-toxicity. The serum metabolic profiles can be used to evaluate the liver-toxicity and to further improve the understanding of underlying mechanism.ObjectiveTo establish the serum metabolic patterns of Erythromycin induced hepatotoxicity in albino wistar rats using 1H NMR based serum metabolomics.ExperimentalFourteen male rats were randomly divided into two groups (n = 7 in each group): control and ERY treated. After 28 days of intervention, the metabolic profiles of sera obtained from ERY and control groups were analyzed using high-resolution 1D 1H CPMG and diffusion-edited nuclear magnetic resonance (NMR) spectra. The histopathological and SEM examinations were employed to evaluate the liver toxicity in ERY treated group.ResultsThe serum metabolic profiles of control and ERY treated rats were compared using multivariate statistical analysis and the metabolic patterns specific to ERY-induced liver toxicity were established. The toxic response of ERY was characterized with: (a) increased serum levels of Glucose, glutamine, dimethylamine, malonate, choline, phosphocholine and phospholipids and (b) decreased levels of isoleucine, leucine, valine, alanine, glutamate, citrate, glycerol, lactate, threonine, circulating lipoproteins, N-acetyl glycoproteins, and poly-unsaturated lipids. These metabolic alterations were found to be associated with (a) decreased TCA cycle activity and enhanced fatty acid oxidation, (b) dysfunction of lipid and amino acid metabolism and (c) oxidative stress.Conclusion and recommendationsErythromycin is often used to produce experimental models of liver toxicity; therefore, the established NMR-based metabolic patterns will form the basis for future studies aiming to evaluate the efficacy of anti-hepatotoxic agents or the hepatotoxicity of new drug-formulations.

Dataset Information

Metabolic effects of basic fibroblast growth factor in streptozotocin-induced diabetic rats: A ¹H NMR-based metabolomics investigation.

Publications

Metabolic effects of basic fibroblast growth factor in streptozotocin-induced diabetic rats: A <sup>1</sup>H NMR-based metabolomics investigation.

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Dataset Information

Metabolic effects of basic fibroblast growth factor in streptozotocin-induced diabetic rats: A 1H NMR-based metabolomics investigation.

Publications

Metabolic effects of basic fibroblast growth factor in streptozotocin-induced diabetic rats: A <sup>1</sup>H NMR-based metabolomics investigation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

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Metabolic effects of basic fibroblast growth factor in streptozotocin-induced diabetic rats: A ¹H NMR-based metabolomics investigation.