Large scale analysis of amino acid substitutions in bacterial proteomics.

ABSTRACT:

Background

Proteomics of bacterial pathogens is a developing field exploring microbial physiology, gene expression and the complex interactions between bacteria and their hosts. One of the complications in proteomic approach is micro- and macro-heterogeneity of bacterial species, which makes it impossible to build a comprehensive database of bacterial genomes for identification, while most of the existing algorithms rely largely on genomic data.

Results

Here we present a large scale study of identification of single amino acid polymorphisms between bacterial strains. An ad hoc method was developed based on MS/MS spectra comparison without the support of a genomic database. Whole-genome sequencing was used to validate the accuracy of polymorphism detection. Several approaches

SUBMITTER: Ischenko D 

PROVIDER: S-EPMC5100282 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature