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Designer nanoscale DNA assemblies programmed from the top down.

ABSTRACT: Scaffolded DNA origami is a versatile means of synthesizing complex molecular architectures. However, the approach is limited by the need to forward-design specific Watson-Crick base pairing manually for any given target structure. Here, we report a general, top-down strategy to design nearly arbitrary DNA architectures autonomously based only on target shape. Objects are represented as closed surfaces rendered as polyhedral networks of parallel DNA duplexes, which enables complete DNA scaffold routing with a spanning tree algorithm. The asymmetric polymerase chain reaction is applied to produce stable, monodisperse assemblies with custom scaffold length and sequence that are verified structurally in three dimensions to be high fidelity by single-particle cryo-electron microscopy. Their long-term stability in serum and low-salt buffer confirms their utility for biological as well as nonbiological applications.

SUBMITTER: Veneziano R

PROVIDER: S-EPMC5111087 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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Designer nanoscale DNA assemblies programmed from the top down.

Veneziano Rémi R Ratanalert Sakul S Zhang Kaiming K Zhang Fei F Yan Hao H Chiu Wah W Bathe Mark M

Science (New York, N.Y.) 20160526 6293

Scaffolded DNA origami is a versatile means of synthesizing complex molecular architectures. However, the approach is limited by the need to forward-design specific Watson-Crick base pairing manually for any given target structure. Here, we report a general, top-down strategy to design nearly arbitrary DNA architectures autonomously based only on target shape. Objects are represented as closed surfaces rendered as polyhedral networks of parallel DNA duplexes, which enables complete DNA scaffold ...[more]

PMID: 27229143

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