Ontology highlight
ABSTRACT:
SUBMITTER: Wei H
PROVIDER: S-EPMC5116584 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Wei Hua H Hu Bo B Tang Suming S Zhao Guojie G Guan Yifu Y
Scientific reports 20161121
Small molecule metabolites and their allosterically regulated repressors play an important role in many gene expression and metabolic disorder processes. These natural sensors, though valuable as good logic switches, have rarely been employed without transcription machinery in cells. Here, two pairs of repressors, which function in opposite ways, were cloned, purified and used to control DNA replication in rolling circle amplification (RCA) in vitro. By using metabolites and repressors as inputs ...[more]