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Single-Cell Mass Cytometry Analysis of the Human Endocrine Pancreas.

ABSTRACT: The human endocrine pancreas consists of multiple cell types and plays a critical role in glucose homeostasis. Here, we apply mass cytometry technology to measure all major islet hormones, proliferative markers, and readouts of signaling pathways involved in proliferation at single-cell resolution. Using this innovative technology, we simultaneously examined baseline proliferation levels of all endocrine cell types from birth through adulthood, as well as in response to the mitogen harmine. High-dimensional analysis of our marker protein expression revealed three major clusters of beta cells within individuals. Proliferating beta cells are confined to two of the clusters.

SUBMITTER: Wang YJ

PROVIDER: S-EPMC5123805 | biostudies-literature | 2016 Oct

REPOSITORIES: biostudies-literature

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Single-Cell Mass Cytometry Analysis of the Human Endocrine Pancreas.

Wang Yue J YJ Golson Maria L ML Schug Jonathan J Traum Daniel D Liu Chengyang C Vivek Kumar K Dorrell Craig C Naji Ali A Powers Alvin C AC Chang Kyong-Mi KM Grompe Markus M Kaestner Klaus H KH

Cell metabolism 20161001 4

The human endocrine pancreas consists of multiple cell types and plays a critical role in glucose homeostasis. Here, we apply mass cytometry technology to measure all major islet hormones, proliferative markers, and readouts of signaling pathways involved in proliferation at single-cell resolution. Using this innovative technology, we simultaneously examined baseline proliferation levels of all endocrine cell types from birth through adulthood, as well as in response to the mitogen harmine. High ...[more]

PMID: 27732837

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Project description:This SuperSeries is composed of the SubSeries listed below.

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