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Continuous single cell imaging reveals sequential steps of plasmacytoid dendritic cell development from common dendritic cell progenitors.

ABSTRACT: Functionally distinct plasmacytoid and conventional dendritic cells (pDC and cDC) shape innate and adaptive immunity. They are derived from common dendritic cell progenitors (CDPs) in the murine bone marrow, which give rise to CD11c⁺ MHCII^- precursors with early commitment to DC subpopulations. In this study, we dissect pDC development from CDP into an ordered sequence of differentiation events by monitoring the expression of CD11c, MHC class II, Siglec H and CCR9 in CDP cultures by continuous single cell imaging and tracking. Analysis of CDP genealogies revealed a stepwise differentiation of CDPs into pDCs in a part of the CDP colonies. This developmental pathway involved an early CD11c⁺ SiglecH^- pre-DC stage and a Siglec H⁺ CCR9^low precursor stage, which was followed rapidly by upregulation of CCR9 indicating final pDC differentiation. In the majority of the remaining CDP pedigrees however the Siglec H⁺ CCR9^low precursor state was maintained for several generations. Thus, although a fraction of CDPs transits through precursor stages rapidly to give rise to a first wave of pDCs, the majority of CDP progeny differentiate more slowly and give rise to longer lived precursor cells which are poised to differentiate on demand.

SUBMITTER: Dursun E

PROVIDER: S-EPMC5124969 | biostudies-literature |

REPOSITORIES: biostudies-literature

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Project description:BACKGROUND:Atopy and viral respiratory tract infections synergistically promote asthma exacerbations. IgE cross-linking inhibits critical virus-induced IFN-? responses of plasmacytoid dendritic cells (pDCs), which can be deficient in patients with allergic asthma. OBJECTIVE:We sought to determine whether reducing IgE levels in vivo with omalizumab treatment increases pDC antiviral IFN-? responses in inner-city children with asthma. METHODS:PBMCs and pDCs isolated from children with exacerbation-prone asthma before and during omalizumab treatment were stimulated ex vivo with rhinovirus and influenza in the presence or absence of IgE cross-linking. IFN-? levels were measured in supernatants, and mRNA expression of IFN-? pathway genes was determined by using quantitative RT-PCR (qRT-PCR) in cell pellets. Fc?RI? protein levels and mRNA expression were measured in unstimulated cells by using flow cytometry and qRT-PCR, respectively. Changes in these outcomes and associations with clinical outcomes were analyzed, and statistical modeling was used to identify risk factors for asthma exacerbations. RESULTS:Omalizumab treatment increased rhinovirus- and influenza-induced PBMC and rhinovirus-induced pDC IFN-? responses in the presence of IgE cross-linking and reduced pDC surface Fc?RI? expression. Omalizumab-induced reductions in pDC Fc?RI? levels were significantly associated with a lower asthma exacerbation rate during the outcome period and correlated with increases in PBMC IFN-? responses. PBMC Fc?RI? mRNA expression measured on study entry significantly improved an existing model of exacerbation prediction. CONCLUSIONS:These findings indicate that omalizumab treatment augments pDC IFN-? responses and attenuates pDC Fc?RI? protein expression and provide evidence that these effects are related. These results support a potential mechanism underlying clinical observations that allergic sensitization is associated with increased susceptibility to virus-induced asthma exacerbations.

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Continuous single cell imaging reveals sequential steps of plasmacytoid dendritic cell development from common dendritic cell progenitors.

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