Engineering dynamical control of cell fate switching using synthetic phospho-regulons.

ABSTRACT: Many cells can sense and respond to time-varying stimuli, selectively triggering changes in cell fate only in response to inputs of a particular duration or frequency. A common motif in dynamically controlled cells is a dual-timescale regulatory network: although long-term fate decisions are ultimately controlled by a slow-timescale switch (e.g., gene expression), input signals are first processed by a fast-timescale signaling layer, which is hypothesized to filter what dynamic information is efficiently relayed downstream. Directly testing the design principles of how dual-timescale circuits control dynamic sensing, however, has been challenging, because most synthetic biology methods have focused solely on rewiring transcriptional circuits, which operate at a single slow timescale. Here,

SUBMITTER: Gordley RM 

PROVIDER: S-EPMC5127309 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature