Ontology highlight
ABSTRACT:
SUBMITTER: Oliveira BL
PROVIDER: S-EPMC5132151 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Oliveira Bruno L BL Guo Zijian Z Boutureira Omar O Guerreiro Ana A Jiménez-Osés Gonzalo G Bernardes Gonçalo J L GJ
Angewandte Chemie (International ed. in English) 20161020 47
The unstrained S-allyl cysteine amino acid was site-specifically installed on apoptosis protein biomarkers and was further used as a chemical handle and ligation partner for 1,2,4,5-tetrazines by means of an inverse-electron-demand Diels-Alder reaction. We demonstrate the utility of this minimal handle for the efficient labeling of apoptotic cells using a fluorogenic tetrazine dye in a pre-targeting approach. The small size, easy chemical installation, and selective reactivity of the S-allyl han ...[more]