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LOVTRAP: an optogenetic system for photoinduced protein dissociation.

ABSTRACT: LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins.

SUBMITTER: Wang H

PROVIDER: S-EPMC5137947 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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LOVTRAP: an optogenetic system for photoinduced protein dissociation.

Wang Hui H Vilela Marco M Winkler Andreas A Tarnawski Miroslaw M Schlichting Ilme I Yumerefendi Hayretin H Kuhlman Brian B Liu Rihe R Danuser Gaudenz G Hahn Klaus M KM

Nature methods 20160718 9

LOVTRAP is an optogenetic approach for reversible light-induced protein dissociation using protein A fragments that bind to the LOV domain only in the dark, with tunable kinetics and a >150-fold change in the dissociation constant (Kd). By reversibly sequestering proteins at mitochondria, we precisely modulated the proteins' access to the cell edge, demonstrating a naturally occurring 3-mHz cell-edge oscillation driven by interactions of Vav2, Rac1, and PI3K proteins. ...[more]

PMID: 27427858

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