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Nanoscale Metal-Organic Frameworks for Ratiometric Oxygen Sensing in Live Cells.

ABSTRACT: We report the design of a phosphorescence/fluorescence dual-emissive nanoscale metal-organic framework (NMOF), R-UiO, as an intracellular oxygen (O2) sensor. R-UiO contains a Pt(II)-porphyrin ligand as an O2-sensitive probe and a Rhodamine-B isothiocyanate ligand as an O2-insensitive reference probe. It exhibits good crystallinity, high stability, and excellent ratiometric luminescence response to O2 partial pressure. In vitro experiments confirmed the applicability of R-UiO as an intracellular O2 biosensor. This work is the first report of a NMOF-based intracellular oxygen sensor and should inspire the design of ratiometric NMOF sensors for other important analytes in biological systems.

SUBMITTER: Xu R

PROVIDER: S-EPMC5139906 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Nanoscale Metal-Organic Frameworks for Ratiometric Oxygen Sensing in Live Cells.

Xu Ruoyu R Wang Youfu Y Duan Xiaopin X Lu Kuangda K Micheroni Daniel D Hu Aiguo A Lin Wenbin W

Journal of the American Chemical Society 20160216 7

We report the design of a phosphorescence/fluorescence dual-emissive nanoscale metal-organic framework (NMOF), R-UiO, as an intracellular oxygen (O2) sensor. R-UiO contains a Pt(II)-porphyrin ligand as an O2-sensitive probe and a Rhodamine-B isothiocyanate ligand as an O2-insensitive reference probe. It exhibits good crystallinity, high stability, and excellent ratiometric luminescence response to O2 partial pressure. In vitro experiments confirmed the applicability of R-UiO as an intracellular ...[more]

PMID: 26864385

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