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Molecular basis for the broad substrate selectivity of a peptide prenyltransferase.


ABSTRACT: The cyanobactin prenyltransferases catalyze a series of known or unprecedented reactions on millions of different substrates, with no easily observable recognition motif and exquisite regioselectivity. Here we define the basis of broad substrate tolerance for the otherwise uncharacterized TruF family. We determined the structures of the Tyr-prenylating enzyme PagF, in complex with an isoprenoid donor analog and a panel of linear and macrocyclic peptide substrates. Unexpectedly, the structures reveal a truncated barrel fold, wherein binding of large peptide substrates is necessary to complete a solvent-exposed hydrophobic pocket to form the catalytically competent active site. Kinetic, mutational, chemical, and computational analyses revealed the structural basis of selectivity, showing a small motif within peptide substrates that is sufficient for recognition by the enzyme. Attaching this 2-residue motif to two random peptides results in their isoprenylation by PagF, demonstrating utility as a general biocatalytic platform for modifications on any peptide substrate.

SUBMITTER: Hao Y 

PROVIDER: S-EPMC5150373 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Molecular basis for the broad substrate selectivity of a peptide prenyltransferase.

Hao Yue Y   Pierce Elizabeth E   Roe Daniel D   Morita Maho M   McIntosh John A JA   Agarwal Vinayak V   Cheatham Thomas E TE   Schmidt Eric W EW   Nair Satish K SK  

Proceedings of the National Academy of Sciences of the United States of America 20161121 49


The cyanobactin prenyltransferases catalyze a series of known or unprecedented reactions on millions of different substrates, with no easily observable recognition motif and exquisite regioselectivity. Here we define the basis of broad substrate tolerance for the otherwise uncharacterized TruF family. We determined the structures of the Tyr-prenylating enzyme PagF, in complex with an isoprenoid donor analog and a panel of linear and macrocyclic peptide substrates. Unexpectedly, the structures re  ...[more]

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