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Digital PCR dynamic range is approaching that of real-time quantitative PCR.

ABSTRACT: Digital PCR (dPCR) has been reported to be more precise and sensitive than real-time quantitative PCR (qPCR) in a variety of models and applications. However, in the majority of commercially available dPCR platforms, the dynamic range is dependent on the number of partitions analysed and so is typically limited to four orders of magnitude; reduced compared with the typical seven orders achievable by qPCR. Using two different biological models (HIV DNA analysis and KRAS genotyping), we have demonstrated that the RainDrop Digital PCR System (RainDance Technologies) is capable of performing accurate and precise quantification over six orders of magnitude thereby approaching that achievable by qPCR.

SUBMITTER: Jones GM

PROVIDER: S-EPMC5154633 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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Digital PCR dynamic range is approaching that of real-time quantitative PCR.

Jones Gerwyn M GM Busby Eloise E Garson Jeremy A JA Grant Paul R PR Nastouli Eleni E Devonshire Alison S AS Whale Alexandra S AS

Biomolecular detection and quantification 20161102

Digital PCR (dPCR) has been reported to be more precise and sensitive than real-time quantitative PCR (qPCR) in a variety of models and applications. However, in the majority of commercially available dPCR platforms, the dynamic range is dependent on the number of partitions analysed and so is typically limited to four orders of magnitude; reduced compared with the typical seven orders achievable by qPCR. Using two different biological models (HIV DNA analysis and <i>KRAS</i> genotyping), we hav ...[more]

PMID: 27990347

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