Ontology highlight
ABSTRACT:
SUBMITTER: Jones GM
PROVIDER: S-EPMC5154633 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Jones Gerwyn M GM Busby Eloise E Garson Jeremy A JA Grant Paul R PR Nastouli Eleni E Devonshire Alison S AS Whale Alexandra S AS
Biomolecular detection and quantification 20161102
Digital PCR (dPCR) has been reported to be more precise and sensitive than real-time quantitative PCR (qPCR) in a variety of models and applications. However, in the majority of commercially available dPCR platforms, the dynamic range is dependent on the number of partitions analysed and so is typically limited to four orders of magnitude; reduced compared with the typical seven orders achievable by qPCR. Using two different biological models (HIV DNA analysis and <i>KRAS</i> genotyping), we hav ...[more]