Ontology highlight
ABSTRACT:
SUBMITTER: Hsu SD
PROVIDER: S-EPMC5159899 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Scientific reports 20161216
Concatenation by covalent linkage of two protomers of an intertwined all-helical HP0242 homodimer from Helicobacter pylori results in the first example of an engineered knotted protein. While concatenation does not affect the native structure according to X-ray crystallography, the folding kinetics is substantially slower compared to the parent homodimer. Using NMR hydrogen-deuterium exchange analysis, we showed here that concatenation destabilises significantly the knotted structure in solution ...[more]