In vivo MEMRI characterization of brain metastases using a 3D Look-Locker T1-mapping sequence.

ABSTRACT: Although MEMRI (Manganese Enhanced MRI) informations were obtained on primary tumors in small animals, MEMRI data on metastases are lacking. Thus, our goal was to determine if 3D Look-Locker T1 mapping was an efficient method to evaluate Mn ions transport in brain metastases in vivo. The high spatial resolution in 3D (156?×?156?×?218??m) of the sequence enabled to detect metastases of 0.3?mm³. In parallel, the T1 quantitation enabled to distinguish three populations of MDA-MB-231 derived brain metastases after MnCl2 intravenous injection: one with a healthy blood-tumor barrier that did not internalize Mn²⁺ ions, and two others, which T1 shortened drastically by 54.2% or 24%. Subsequent scans of the mice, enabled by the fast acquisition (23?min), demonstrated that these T1 reached back their pre-injection values in 24?h. Contrarily to metastases, the T1 of U87-MG glioma remained 26.2% shorter for one week. In vitro results supported the involvement of the Transient Receptor Potential channels and the Calcium-Sensing Receptor in the uptake and efflux of Mn²⁺ ions, respectively. This study highlights the ability of the 3D Look-Locker T1 mapping sequence to study heterogeneities (i) amongst brain metastases and (ii) between metastases and glioma regarding Mn transport.

SUBMITTER: Castets CR 

PROVIDER: S-EPMC5171659 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature