Coupled ATPase-adenylate kinase activity in ABC transporters.
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ABSTRACT:
SUBMITTER: Kaur H
PROVIDER: S-EPMC5192220 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Publications
Coupled ATPase-adenylate kinase activity in ABC transporters.
Kaur Hundeep H Lakatos-Karoly Andrea A Vogel Ramona R Nöll Anne A Tampé Robert R Glaubitz Clemens C
Nature communications 20161222
ATP-binding cassette (ABC) transporters, a superfamily of integral membrane proteins, catalyse the translocation of substrates across the cellular membrane by ATP hydrolysis. Here we demonstrate by nucleotide turnover and binding studies based on <sup>31</sup>P solid-state NMR spectroscopy that the ABC exporter and lipid A flippase MsbA can couple ATP hydrolysis to an adenylate kinase activity, where ADP is converted into AMP and ATP. Single-point mutations reveal that both ATPase and adenylate ...[more]
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