Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics.
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ABSTRACT:
SUBMITTER: Wang S
PROVIDER: S-EPMC5216389 | biostudies-literature | 2017 Jan
REPOSITORIES: biostudies-literature
Publications
Non-invasive, Focused Ultrasound-Facilitated Gene Delivery for Optogenetics.
Wang Shutao S Kugelman Tara T Buch Amanda A Herman Mathieu M Han Yang Y Karakatsani Maria Eleni ME Hussaini S Abid SA Duff Karen K Konofagou Elisa E EE
Scientific reports 20170106
Optogenetics, a widely used technique in neuroscience research, is often limited by its invasive nature of application. Here, we present a noninvasive, ultrasound-based technique to introduce optogenetic channels into the brain by temporarily opening the blood-brain barrier (BBB). We demonstrate the efficiency of the method developed and evaluate the bioactivity of the non-invasively introduced channelrhodopsin channels by performing stimulation in freely behaving mice. ...[more]
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