Unknown

Dataset Information

0

Isomerically Pure Tetramethylrhodamine Voltage Reporters.


ABSTRACT: We present the design, synthesis, and application of a new family of fluorescent voltage indicators based on isomerically pure tetramethylrhodamines. These new Rhodamine Voltage Reporters, or RhoVRs, use photoinduced electron transfer (PeT) as a trigger for voltage sensing, display excitation and emission profiles in the green to orange region of the visible spectrum, demonstrate high sensitivity to membrane potential changes (up to 47% ?F/F per 100 mV), and employ a tertiary amide derived from sarcosine, which aids in membrane localization and simultaneously simplifies the synthetic route to the voltage sensors. The most sensitive of the RhoVR dyes, RhoVR 1, features a methoxy-substituted diethylaniline donor and phenylenevinylene molecular wire at the 5'-position of the rhodamine aryl ring, exhibits the highest voltage sensitivity to date for red-shifted PeT-based voltage sensors, and is compatible with simultaneous imaging alongside green fluorescent protein-based indicators. The discoveries that sarcosine-based tertiary amides in the context of molecular-wire voltage indicators prevent dye internalization and 5'-substituted voltage indicators exhibit improved voltage sensitivity should be broadly applicable to other types of PeT-based voltage-sensitive fluorophores.

SUBMITTER: Deal PE 

PROVIDER: S-EPMC5222532 | biostudies-literature | 2016 Jul

REPOSITORIES: biostudies-literature

altmetric image

Publications

Isomerically Pure Tetramethylrhodamine Voltage Reporters.

Deal Parker E PE   Kulkarni Rishikesh U RU   Al-Abdullatif Sarah H SH   Miller Evan W EW  

Journal of the American Chemical Society 20160718 29


We present the design, synthesis, and application of a new family of fluorescent voltage indicators based on isomerically pure tetramethylrhodamines. These new Rhodamine Voltage Reporters, or RhoVRs, use photoinduced electron transfer (PeT) as a trigger for voltage sensing, display excitation and emission profiles in the green to orange region of the visible spectrum, demonstrate high sensitivity to membrane potential changes (up to 47% ΔF/F per 100 mV), and employ a tertiary amide derived from  ...[more]

Similar Datasets

| S-EPMC2919757 | biostudies-literature
| S-EPMC5947138 | biostudies-literature
| S-EPMC3419450 | biostudies-other
| S-EPMC7304281 | biostudies-literature
| S-EPMC4134104 | biostudies-literature
| S-EPMC5866759 | biostudies-literature
| S-EPMC6949409 | biostudies-literature
| S-EPMC4624957 | biostudies-literature
| S-EPMC6954400 | biostudies-literature
| S-EPMC9794836 | biostudies-literature