Ontology highlight
ABSTRACT:
SUBMITTER: Levendosky RF
PROVIDER: S-EPMC5226652 | biostudies-literature | 2016 Dec
REPOSITORIES: biostudies-literature
Levendosky Robert F RF Sabantsev Anton A Deindl Sebastian S Bowman Gregory D GD
eLife 20161229
Despite their canonical two-fold symmetry, nucleosomes in biological contexts are often asymmetric: functionalized with post-translational modifications (PTMs), substituted with histone variants, and even lacking H2A/H2B dimers. Here we show that the Widom 601 nucleosome positioning sequence can produce hexasomes in a specific orientation on DNA, providing a useful tool for interrogating chromatin enzymes and allowing for the generation of nucleosomes with precisely defined asymmetry. Using this ...[more]