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Improved Detection of Respiratory Pathogens by Use of High-Quality Sputum with TaqMan Array Card Technology.


ABSTRACT: New diagnostic platforms often use nasopharyngeal or oropharyngeal (NP/OP) swabs for pathogen detection for patients hospitalized with community-acquired pneumonia (CAP). We applied multipathogen testing to high-quality sputum specimens to determine if more pathogens can be identified relative to NP/OP swabs. Children (<18 years old) and adults hospitalized with CAP were enrolled over 2.5 years through the Etiology of Pneumonia in the Community (EPIC) study. NP/OP specimens with matching high-quality sputum (defined as ?10 epithelial cells/low-power field [lpf] and ?25 white blood cells/lpf or a quality score [q-score] definition of 2+) were tested by TaqMan array card (TAC), a multipathogen real-time PCR detection platform. Among 236 patients with matched specimens, a higher proportion of sputum specimens had ?1 pathogen detected compared with NP/OP specimens in children (93% versus 68%; P < 0.0001) and adults (88% versus 61%; P < 0.0001); for each pathogen targeted, crossing threshold (CT) values were earlier in sputum. Both bacterial (361 versus 294) and viral detections (245 versus 140) were more common in sputum versus NP/OP specimens, respectively, in both children and adults. When available, high-quality sputum may be useful for testing in hospitalized CAP patients.

SUBMITTER: Wolff BJ 

PROVIDER: S-EPMC5228222 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Improved Detection of Respiratory Pathogens by Use of High-Quality Sputum with TaqMan Array Card Technology.

Wolff Bernard J BJ   Bramley Anna M AM   Thurman Kathleen A KA   Whitney Cynthia G CG   Whitaker Brett B   Self Wesley H WH   Arnold Sandra R SR   Trabue Christopher C   Wunderink Richard G RG   McCullers Jon J   Edwards Kathryn M KM   Jain Seema S   Winchell Jonas M JM  

Journal of clinical microbiology 20161228 1


New diagnostic platforms often use nasopharyngeal or oropharyngeal (NP/OP) swabs for pathogen detection for patients hospitalized with community-acquired pneumonia (CAP). We applied multipathogen testing to high-quality sputum specimens to determine if more pathogens can be identified relative to NP/OP swabs. Children (<18 years old) and adults hospitalized with CAP were enrolled over 2.5 years through the Etiology of Pneumonia in the Community (EPIC) study. NP/OP specimens with matching high-qu  ...[more]

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