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Comparative analytical evaluation of the respiratory TaqMan Array Card with real-time PCR and commercial multi-pathogen assays.


ABSTRACT: In this study, a multicenter evaluation of the Life Technologies TaqMan(®) Array Card (TAC) with 21 custom viral and bacterial respiratory assays was performed on the Applied Biosystems ViiA™ 7 Real-Time PCR System. The goal of the study was to demonstrate the analytical performance of this platform when compared to identical individual pathogen specific laboratory developed tests (LDTs) designed at the Centers for Disease Control and Prevention (CDC), equivalent LDTs provided by state public health laboratories, or to three different commercial multi-respiratory panels. CDC and Association of Public Health Laboratories (APHL) LDTs had similar analytical sensitivities for viral pathogens, while several of the bacterial pathogen APHL LDTs demonstrated sensitivities one log higher than the corresponding CDC LDT. When compared to CDC LDTs, TAC assays were generally one to two logs less sensitive depending on the site performing the analysis. Finally, TAC assays were generally more sensitive than their counterparts in three different commercial multi-respiratory panels. TAC technology allows users to spot customized assays and design TAC layout, simplify assay setup, conserve specimen, dramatically reduce contamination potential, and as demonstrated in this study, analyze multiple samples in parallel with good reproducibility between instruments and operators.

SUBMITTER: Harvey JJ 

PROVIDER: S-EPMC7113746 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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Comparative analytical evaluation of the respiratory TaqMan Array Card with real-time PCR and commercial multi-pathogen assays.

Harvey John J JJ   Chester Stephanie S   Burke Stephen A SA   Ansbro Marisela M   Aden Tricia T   Gose Remedios R   Sciulli Rebecca R   Bai Jing J   DesJardin Lucy L   Benfer Jeffrey L JL   Hall Joshua J   Smole Sandra S   Doan Kimberly K   Popowich Michael D MD   St George Kirsten K   Quinlan Tammy T   Halse Tanya A TA   Li Zhen Z   Pérez-Osorio Ailyn C AC   Glover William A WA   Russell Denny D   Reisdorf Erik E   Whyte Thomas T   Whitaker Brett B   Hatcher Cynthia C   Srinivasan Velusamy V   Tatti Kathleen K   Tondella Maria Lucia ML   Wang Xin X   Winchell Jonas M JM   Mayer Leonard W LW   Jernigan Daniel D   Mawle Alison C AC  

Journal of virological methods 20151127


In this study, a multicenter evaluation of the Life Technologies TaqMan(®) Array Card (TAC) with 21 custom viral and bacterial respiratory assays was performed on the Applied Biosystems ViiA™ 7 Real-Time PCR System. The goal of the study was to demonstrate the analytical performance of this platform when compared to identical individual pathogen specific laboratory developed tests (LDTs) designed at the Centers for Disease Control and Prevention (CDC), equivalent LDTs provided by state public he  ...[more]

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