Regulation of the endogenous VEGF-A gene by exogenous designed regulatory proteins.
Ontology highlight
ABSTRACT:
SUBMITTER: Tachikawa K
PROVIDER: S-EPMC523457 | biostudies-literature | 2004 Oct
REPOSITORIES: biostudies-literature
Publications
Regulation of the endogenous VEGF-A gene by exogenous designed regulatory proteins.
Tachikawa Kiyoshi K Schröder Oliver O Frey Gerhard G Briggs Steven P SP Sera Takashi T
Proceedings of the National Academy of Sciences of the United States of America 20041008 42
We describe a facile method to activate or repress transcription of endogenous genes in a quantitative and specific manner by treatment with designed regulatory proteins (DRPs), in which artificial transcription factors (ATFs) are fused to cell-penetrating peptides (CPPs). Penetration of DRPs into cells is mediated by an N-terminal CPP fused to a nuclear localization signal; a DNA-binding domain and a transactivation domain follow. The DNA-binding domain was targeted to the vascular endothelial ...[more]
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