Ontology highlight
ABSTRACT:
SUBMITTER: Redchuk TA
PROVIDER: S-EPMC6992714 | biostudies-literature | 2020 Jan
REPOSITORIES: biostudies-literature
Redchuk Taras A TA Karasev Maksim M MM Verkhusha Polina V PV Donnelly Sara K SK Hülsemann Maren M Virtanen Jori J Moore Henna M HM Vartiainen Maria K MK Hodgson Louis L Verkhusha Vladislav V VV
Nature communications 20200130 1
Techniques of protein regulation, such as conditional gene expression, RNA interference, knock-in and knock-out, lack sufficient spatiotemporal accuracy, while optogenetic tools suffer from non-physiological response due to overexpression artifacts. Here we present a near-infrared light-activatable optogenetic system, which combines the specificity and orthogonality of intrabodies with the spatiotemporal precision of optogenetics. We engineer optically-controlled intrabodies to regulate genomica ...[more]