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An expanded nuclear phylogenomic PCR toolkit for Sapindales.

ABSTRACT: PREMISE OF THE STUDY:We tested PCR amplification of 91 low-copy nuclear gene loci in taxa from Sapindales using primers developed for Bursera simaruba (Burseraceae). METHODS AND RESULTS:Cross-amplification of these markers among 10 taxa tested was related to their phylogenetic distance from B. simaruba. On average, each Sapindalean taxon yielded product for 53 gene regions (range: 16-90). Arabidopsis thaliana (Brassicales), by contrast, yielded product for two. Single representatives of Anacardiaceae and Rutacaeae yielded 34 and 26 products, respectively. Twenty-six primer pairs worked for all Burseraceae species tested if highly divergent Aucoumea klaineana is excluded, and eight of these amplified product in every Sapindalean taxon. CONCLUSIONS:Our study demonstrates that customized primers for Bursera can amplify product in a range of Sapindalean taxa. This collection of primer pairs, therefore, is a valuable addition to the toolkit for nuclear phylogenomic analyses of Sapindales and warrants further investigation.

SUBMITTER: Collins ES

PROVIDER: S-EPMC5238699 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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An expanded nuclear phylogenomic PCR toolkit for Sapindales.

Collins Elizabeth S ES Gostel Morgan R MR Weeks Andrea A

Applications in plant sciences 20161207 12

<h4>Premise of the study</h4>We tested PCR amplification of 91 low-copy nuclear gene loci in taxa from Sapindales using primers developed for <i>Bursera simaruba</i> (Burseraceae).<h4>Methods and results</h4>Cross-amplification of these markers among 10 taxa tested was related to their phylogenetic distance from <i>B. simaruba</i>. On average, each Sapindalean taxon yielded product for 53 gene regions (range: 16-90). <i>Arabidopsis thaliana</i> (Brassicales), by contrast, yielded product for two ...[more]

PMID: 28101434

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