Ontology highlight
ABSTRACT:
SUBMITTER: Nolen BJ
PROVIDER: S-EPMC524860 | biostudies-literature | 2004 Nov
REPOSITORIES: biostudies-literature
Nolen Brad J BJ Littlefield Ryan S RS Pollard Thomas D TD
Proceedings of the National Academy of Sciences of the United States of America 20041025 44
Actin-related protein (Arp) 2/3 complex stimulates formation of actin filaments at the leading edge of motile cells. Nucleation of filaments depends on hydrolysis of ATP bound to Arp2. Here we report crystal structures of Arp2/3 complex with bound ATP or ADP. The nucleotides are immobilized on the face of subdomains 3 and 4 of Arp2, whereas subdomains 1 and 2 are flexible and absent from the electron density maps. This flexibility may explain why Arp2 does not hydrolyze ATP until the complex is ...[more]