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CASCADE, a platform for controlled gene amplification for high, tunable and selection-free gene expression in yeast.

ABSTRACT: Over-expression of a gene by increasing its copy number is often desirable in the model yeast Saccharomyces cerevisiae. It may facilitate elucidation of enzyme functions, and in cell factory design it is used to increase production of proteins and metabolites. Current methods are typically exploiting expression from the multicopy 2??-derived plasmid or by targeting genes repeatedly into sequences like Ty or rDNA; in both cases, high gene expression levels are often reached. However, with 2??-based plasmid expression, the population of cells is very heterogeneous with respect to protein production; and for integration into repeated sequences it is difficult to determine the genetic setup of the resulting strains and to achieve specific gene doses. For both types of systems, the strains often suffer from genetic instability if proper selection pressure is not applied. Here we present a gene amplification system, CASCADE, which enables construction of strains with defined gene copy numbers. One or more genes can be amplified simultaneously and the resulting strains can be stably propagated on selection-free medium. As proof-of-concept, we have successfully used CASCADE to increase heterologous production of two fluorescent proteins, the enzyme ?-galactosidase the fungal polyketide 6-methyl salicylic acid and the plant metabolite vanillin glucoside.

SUBMITTER: Strucko T

PROVIDER: S-EPMC5278378 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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CASCADE, a platform for controlled gene amplification for high, tunable and selection-free gene expression in yeast.

Strucko Tomas T Buron Line Due LD Jarczynska Zofia Dorota ZD Nødvig Christina Spuur CS Mølgaard Louise L Halkier Barbara Ann BA Mortensen Uffe Hasbro UH

Scientific reports 20170130

Over-expression of a gene by increasing its copy number is often desirable in the model yeast Saccharomyces cerevisiae. It may facilitate elucidation of enzyme functions, and in cell factory design it is used to increase production of proteins and metabolites. Current methods are typically exploiting expression from the multicopy 2 μ-derived plasmid or by targeting genes repeatedly into sequences like Ty or rDNA; in both cases, high gene expression levels are often reached. However, with 2 μ-bas ...[more]

PMID: 28134264

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Dataset Information

CASCADE, a platform for controlled gene amplification for high, tunable and selection-free gene expression in yeast.

Publications

CASCADE, a platform for controlled gene amplification for high, tunable and selection-free gene expression in yeast.

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