Ontology highlight
ABSTRACT:
SUBMITTER: McIsaac RS
PROVIDER: S-EPMC3575806 | biostudies-other | 2013 Feb
REPOSITORIES: biostudies-other
McIsaac R Scott RS Oakes Benjamin L BL Wang Xin X Dummit Krysta A KA Botstein David D Noyes Marcus B MB
Nucleic acids research 20121228 4
A general method for the dynamic control of single gene expression in eukaryotes, with no off-target effects, is a long-sought tool for molecular and systems biologists. We engineered two artificial transcription factors (ATFs) that contain Cys(2)His(2) zinc-finger DNA-binding domains of either the mouse transcription factor Zif268 (9 bp of specificity) or a rationally designed array of four zinc fingers (12 bp of specificity). These domains were expressed as fusions to the human estrogen recept ...[more]