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Single dish gradient screening of small molecule localization.

ABSTRACT: Understanding trafficking in cells and tissues is one of the most critical steps in exploring the mechanisms and modes of action (MOAs) of a small molecule. Typically, deciphering the role of concentration presents one of the most difficult challenges associated with this task. Herein, we present a practical solution to this problem by developing concentration gradients within single dishes of cells. We demonstrate the method by evaluating fluorescently-labelled probes developed from two classes of natural products that have been identified as potential anti-cancer leads by STORM super-resolution microscopy.

SUBMITTER: Beuzer P

PROVIDER: S-EPMC5284121 | biostudies-literature | 2016 Sep

REPOSITORIES: biostudies-literature

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Single dish gradient screening of small molecule localization.

Beuzer Paolo P Axelrod Joshua J Trzoss Lynnie L Fenical Willam W Dasari Ramesh R Evidente Antonio A Kornienko Alexander A Cang Hu H La Clair James J JJ

Organic & biomolecular chemistry 20160817 35

Understanding trafficking in cells and tissues is one of the most critical steps in exploring the mechanisms and modes of action (MOAs) of a small molecule. Typically, deciphering the role of concentration presents one of the most difficult challenges associated with this task. Herein, we present a practical solution to this problem by developing concentration gradients within single dishes of cells. We demonstrate the method by evaluating fluorescently-labelled probes developed from two classes ...[more]

PMID: 27530345

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