The HOOK region of voltage-gated Ca2+ channel β subunits senses and transmits PIP2 signals to the gate.

ABSTRACT: The β subunit of voltage-gated Ca²⁺ (Ca_V) channels plays an important role in regulating gating of the α1 pore-forming subunit and its regulation by phosphatidylinositol 4,5-bisphosphate (PIP₂). Subcellular localization of the Ca_V β subunit is critical for this effect; N-terminal-dependent membrane targeting of the β subunit slows inactivation and decreases PIP₂ sensitivity. Here, we provide evidence that the HOOK region of the β subunit plays an important role in the regulation of Ca_V biophysics. Based on amino acid composition, we broadly divide the HOOK region into three domains: S (polyserine), A (polyacidic), and B (polybasic). We show that a β subunit containing only its A domain in the HOOK region increases inactivati

SUBMITTER: Park CG 

PROVIDER: S-EPMC5299622 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature