Ontology highlight
ABSTRACT:
SUBMITTER: Peck SC
PROVIDER: S-EPMC5302023 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Peck Spencer C SC Wang Chen C Dassama Laura M K LM Zhang Bo B Guo Yisong Y Rajakovich Lauren J LJ Bollinger J Martin JM Krebs Carsten C van der Donk Wilfred A WA
Journal of the American Chemical Society 20170131 5
Activation of O-H bonds by inorganic metal-oxo complexes has been documented, but no cognate enzymatic process is known. Our mechanistic analysis of 2-hydroxyethylphosphonate dioxygenase (HEPD), which cleaves the C1-C2 bond of its substrate to afford hydroxymethylphosphonate on the biosynthetic pathway to the commercial herbicide phosphinothricin, uncovered an example of such an O-H-bond-cleavage event. Stopped-flow UV-visible absorption and freeze-quench Mössbauer experiments identified a trans ...[more]