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?-Synuclein increases ?-amyloid secretion by promoting ?-/?-secretase processing of APP.


ABSTRACT: ?-Synuclein misfolding and aggregation is often accompanied by ?-amyloid deposition in some neurodegenerative diseases. We hypothesised that ?-synuclein promotes ?-amyloid production from APP. ?-Amyloid levels and APP amyloidogenic processing were investigated in neuronal cell lines stably overexpressing wildtype and mutant ?-synuclein. ?-Secretase activity and ?-secretase expression were also measured. We show that ?-synuclein expression induces ?-amyloid secretion and amyloidogenic processing of APP in neuronal cell lines. Certain mutations of ?-synuclein potentiate APP amyloidogenic processing. ?-Secretase activity was not enhanced by wildtype ?-synuclein expression, however ?-secretase protein levels were induced. Furthermore, a correlation between ?-synuclein and ?-secretase protein was seen in rat brain striata. Iron chelation abolishes the effect of ?-synuclein on neuronal cell ?-amyloid secretion, whereas overexpression of the ferrireductase enzyme Steap3 is robustly pro-amyloidogenic. We propose that ?-synuclein promotes ?-amyloid formation by modulating ?-cleavage of APP, and that this is potentially mediated by the levels of reduced iron and oxidative stress.

SUBMITTER: Roberts HL 

PROVIDER: S-EPMC5302447 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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α-Synuclein increases β-amyloid secretion by promoting β-/γ-secretase processing of APP.

Roberts Hazel L HL   Schneider Bernard L BL   Brown David R DR  

PloS one 20170210 2


α-Synuclein misfolding and aggregation is often accompanied by β-amyloid deposition in some neurodegenerative diseases. We hypothesised that α-synuclein promotes β-amyloid production from APP. β-Amyloid levels and APP amyloidogenic processing were investigated in neuronal cell lines stably overexpressing wildtype and mutant α-synuclein. γ-Secretase activity and β-secretase expression were also measured. We show that α-synuclein expression induces β-amyloid secretion and amyloidogenic processing  ...[more]

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