CCL2 nitration is a negative regulator of chemokine-mediated inflammation.

ABSTRACT: Chemokines promote leukocyte recruitment during inflammation. The oxidative burst is an important effector mechanism, this leads to the generation of reactive nitrogen species (RNS), including peroxynitrite (ONOO). The current study was performed to determine the potential for nitration to alter the chemical and biological properties of the prototypical CC chemokine, CCL2. Immunofluorescence was performed to assess the presence of RNS in kidney biopsies. Co-localisation was observed between RNS-modified tyrosine residues and the chemokine CCL2 in diseased kidneys. Nitration reduced the potential of CCL2 to stimulate monocyte migration in diffusion gradient chemotaxis assays (p?

SUBMITTER: Barker CE 

PROVIDER: S-EPMC5349559 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature