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Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4.

ABSTRACT: The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively.

SUBMITTER: Ito M

PROVIDER: S-EPMC535301 | biostudies-literature | 2004 Dec

REPOSITORIES: biostudies-literature

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Development and evaluation of fluorogenic TaqMan reverse transcriptase PCR assays for detection of dengue virus types 1 to 4.

Ito Mikako M Takasaki Tomohiko T Yamada Ken-Ichiro K Nerome Reiko R Tajima Shigeru S Kurane Ichiro I

Journal of clinical microbiology 20041201 12

The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively. ...[more]

PMID: 15583346

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