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Design of a synthetic luminescent probe from a biomolecule binding domain: selective detection of AU-rich mRNA sequences.

ABSTRACT: We report the design of a luminescent sensor based upon the zinc finger (ZF) protein TIS11d, that allows for the selective time-resolved detection of the UUAUUUAUU sequence of the 3'-untranslated region of messenger RNA. This sensor is composed of the tandem ZF RNA binding domain of TIS11d functionalized with a luminescent Tb3+ complex on one of the ZFs and a sensitizing antenna on the other. This work provides the proof of principle that an RNA binding protein can be re-engineered as an RNA sensor and, more generally, that tunable synthetic luminescent probes for biomolecules can be obtained by modifying biomolecule-binding domains.

SUBMITTER: Raibaut L

PROVIDER: S-EPMC5364516 | biostudies-literature | 2017 Feb

REPOSITORIES: biostudies-literature

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Design of a synthetic luminescent probe from a biomolecule binding domain: selective detection of AU-rich mRNA sequences.

Raibaut Laurent L Vasseur William W Shimberg Geoffrey D GD Saint-Pierre Christine C Ravanat Jean-Luc JL Michel Sarah L J SLJ Sénèque Olivier O

Chemical science 20161116 2

We report the design of a luminescent sensor based upon the zinc finger (ZF) protein TIS11d, that allows for the selective time-resolved detection of the UUAUUUAUU sequence of the 3'-untranslated region of messenger RNA. This sensor is composed of the tandem ZF RNA binding domain of TIS11d functionalized with a luminescent Tb<sup>3+</sup> complex on one of the ZFs and a sensitizing antenna on the other. This work provides the proof of principle that an RNA binding protein can be re-engineered as ...[more]

PMID: 28451295

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2017-01-01 | GSE66155 | GEO

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