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Crystal structure of enolase from Drosophila melanogaster.

ABSTRACT: Enolase is an important enzyme in glycolysis and various biological processes. Its dysfunction is closely associated with diseases. Here, the enolase from Drosophila melanogaster (DmENO) was purified and crystallized. A crystal of DmENO diffracted to 2.0?Å resolution and belonged to space group R32. The structure was solved by molecular replacement. Like most enolases, DmENO forms a homodimer with conserved residues in the dimer interface. DmENO possesses an open conformation in this structure and contains conserved elements for catalytic activity. This work provides a structural basis for further functional and evolutionary studies of enolase.

SUBMITTER: Sun C

PROVIDER: S-EPMC5379173 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Crystal structure of enolase from Drosophila melanogaster.

Sun Congcong C Xu Baokui B Liu Xueyan X Zhang Zhen Z Su Zhongliang Z

Acta crystallographica. Section F, Structural biology communications 20170322 Pt 4

Enolase is an important enzyme in glycolysis and various biological processes. Its dysfunction is closely associated with diseases. Here, the enolase from Drosophila melanogaster (DmENO) was purified and crystallized. A crystal of DmENO diffracted to 2.0 Å resolution and belonged to space group R32. The structure was solved by molecular replacement. Like most enolases, DmENO forms a homodimer with conserved residues in the dimer interface. DmENO possesses an open conformation in this structure a ...[more]

PMID: 28368282

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