Ontology highlight
ABSTRACT:
SUBMITTER: Neves MAD
PROVIDER: S-EPMC5388413 | biostudies-literature | 2017 Feb
REPOSITORIES: biostudies-literature
Neves Miguel A D MAD Slavkovic Sladjana S Churcher Zachary R ZR Johnson Philip E PE
Nucleic acids research 20170201 3
Multisite ligand binding by proteins is commonly utilized in the regulation of biological systems and exploited in a range of biochemical technologies. Aptamers, although widely utilized in many rationally designed biochemical systems, are rarely capable of multisite ligand binding. The cocaine-binding aptamer is often used for studying and developing sensor and aptamer-based technologies. Here, we use isothermal titration calorimetry (ITC) and NMR spectroscopy to demonstrate that the cocaine-bi ...[more]