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CNBP acts as a key transcriptional regulator of sustained expression of interleukin-6.


ABSTRACT: The transcription of inflammatory genes is an essential step in host defense activation. Here, we show that cellular nucleic acid-binding protein (CNBP) acts as a transcription regulator that is required for activating the innate immune response. We identified specific CNBP-binding motifs present in the promoter region of sustained inflammatory cytokines, thus, directly inducing the expression of target genes. In particular, lipopolysaccharide (LPS) induced cnbp expression through an NF-?B-dependent manner and a positive autoregulatory mechanism, which enables prolonged il-6 gene expression. This event depends strictly on LPS-induced CNBP nuclear translocation through phosphorylation-mediated dimerization. Consequently, cnbp-depleted zebrafish are highly susceptible to Shigella flexneri infection in vivo. Collectively, these observations identify CNBP as a key transcriptional regulator required for activating and maintaining the immune response.

SUBMITTER: Lee E 

PROVIDER: S-EPMC5389554 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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CNBP acts as a key transcriptional regulator of sustained expression of interleukin-6.

Lee Eunhye E   Lee Taeyun A TA   Kim Ji Hyun JH   Park Areum A   Ra Eun A EA   Kang Sujin S   Choi Hyun Jin HJ   Choi Junhee L JL   Huh Hyunbin D HD   Lee Ji Eun JE   Lee Sungwook S   Park Boyoun B  

Nucleic acids research 20170401 6


The transcription of inflammatory genes is an essential step in host defense activation. Here, we show that cellular nucleic acid-binding protein (CNBP) acts as a transcription regulator that is required for activating the innate immune response. We identified specific CNBP-binding motifs present in the promoter region of sustained inflammatory cytokines, thus, directly inducing the expression of target genes. In particular, lipopolysaccharide (LPS) induced cnbp expression through an NF-κB-depen  ...[more]

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