Ontology highlight
ABSTRACT:
SUBMITTER: Lee YK
PROVIDER: S-EPMC5391143 | biostudies-literature | 2017 Apr
REPOSITORIES: biostudies-literature
Lee You-Kyung YK Jun Yong-Woo YW Choi Ha-Eun HE Huh Yang Hoon YH Kaang Bong-Kiun BK Jang Deok-Jin DJ Lee Jin-A JA
The EMBO journal 20170320 8
Macroautophagy allows for bulk degradation of cytosolic components in lysosomes. Overexpression of GFP/RFP-LC3/GABARAP is commonly used to monitor autophagosomes, a hallmark of autophagy, despite artifacts related to their overexpression. Here, we developed new sensors that detect endogenous LC3/GABARAP proteins at the autophagosome using an LC3-interacting region (LIR) and a short hydrophobic domain (HyD). Among HyD-LIR-GFP sensors harboring LIR motifs of 34 known LC3-binding proteins, HyD-LIR( ...[more]