Dataset Information

Effect of captopril on radiation-induced TGF-?1 secretion in EA.Hy926 human umbilical vein endothelial cells.

ABSTRACT: The pathophysiological mechanism involved in the sustained endothelial secretion of cytokines that leads to fibrosis 6-16 months after radiotherapy remains unclear. Angiotensin II (Ang II) is produced by the endothelium in response to stressing stimuli, like radiation, and may induce the synthesis of TGF-?, a profibrotic cytokine. In this study we tested the hypothesis that captopril, an angiotensin-converting enzyme (ACE) inhibitor, inhibits or attenuates radiation-induced endothelial TGF-?1 secretion. The human endothelial hybrid cell line EA.HY926 was irradiated with split doses of x-rays (28 Gy delivered in 14 fractions of 2 Gy). TGF-?1 mRNA, TNF-? mRNA and TGF-?1 protein levels were evaluated by RT-PCR and western blotting each month until the fifth month post radiation. Ang II was detected using radioimmunoassays, NF-?B activity was examined using EMSA, and western blotting was used to detect the expression of I?-B?. To explore the role of Ang II on radiation-induced TGF-?1 release and I?-B? expression, captopril was added to cultured cells before, during, or after irradiation. Sustained strong expression of TGF-?1 was observed after conventional fractionated irradiation. TNF-?, Ang II, and NF-?B activity were also increased in EA.Hy926 cells after radiation. Captopril decreased Ang II expression, inhibited the NF-?B pathway and reduced TGF-?1 expression. These data suggest that captopril might protect the endothelium from radiation-induced injury.

SUBMITTER: Wei J

PROVIDER: S-EPMC5400550 | biostudies-literature | 2017 Mar

REPOSITORIES: biostudies-literature

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Effect of captopril on radiation-induced TGF-β1 secretion in EA.Hy926 human umbilical vein endothelial cells.

Wei Jingni J Xu Hui H Liu Yinyin Y Li Baiyu B Zhou Fuxiang F

Oncotarget 20170301 13

The pathophysiological mechanism involved in the sustained endothelial secretion of cytokines that leads to fibrosis 6-16 months after radiotherapy remains unclear. Angiotensin II (Ang II) is produced by the endothelium in response to stressing stimuli, like radiation, and may induce the synthesis of TGF-β, a profibrotic cytokine. In this study we tested the hypothesis that captopril, an angiotensin-converting enzyme (ACE) inhibitor, inhibits or attenuates radiation-induced endothelial TGF-β1 se ...[more]

PMID: 28209920

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Dataset Information

Effect of captopril on radiation-induced TGF-?1 secretion in EA.Hy926 human umbilical vein endothelial cells.

Publications

Effect of captopril on radiation-induced TGF-β1 secretion in EA.Hy926 human umbilical vein endothelial cells.

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