Unknown

Dataset Information

0

A generic 89Zr labeling method to quantify the in vivo pharmacokinetics of liposomal nanoparticles with positron emission tomography.


ABSTRACT: Liposomal nanoparticles are versatile drug delivery vehicles that show great promise in cancer therapy. In an effort to quantitatively measure their in vivo pharmacokinetics, we developed a highly efficient 89Zr liposome-labeling method based on a rapid ligand exchange reaction between the membrane-permeable 89Zr(8-hydroxyquinolinate)4 complex and the hydrophilic liposomal cavity-encapsulated deferoxamine (DFO). This novel 89Zr-labeling strategy allowed us to prepare radiolabeled forms of a folic acid (FA)-decorated active targeting 89Zr-FA-DFO-liposome, a thermosensitive 89Zr-DFO-liposome, and a renal avid 89Zr-PEG-DFO-liposome at room temperature with near-quantitative isolated radiochemical yields of 98%±1% (n=6), 98%±2% (n=5), and 97%±1% (n=3), respectively. These 89Zr-labeled liposomal nanoparticles showed remarkable stability in phosphate-buffered saline and serum at 37°C without leakage of radioactivity for 48 h. The uptake of 89Zr-FA-DFO-liposome by the folate receptor-overexpressing KB cells was almost 15-fold higher than the 89Zr-DFO-liposome in vitro. Positron emission tomography imaging and ex vivo biodistribution studies enabled us to observe the heterogeneous distribution of the 89Zr-FA-DFO-liposome and 89Zr-DFO-liposome in the KB tumor xenografts, the extensive kidney accumulation of the 89Zr-FA-DFO-liposome and 89Zr-PEG-DFO-liposome, and the different metabolic fate of the free and liposome-encapsulated 89Zr-DFO. It also unveiled the poor resistance of all three liposomes against endothelial uptake resulting in their catabolism and high uptake of free 89Zr in the skeleton. Thus, this technically simple 89Zr-labeling method would find widespread use to guide the development and clinical applications of novel liposomal nanomedicines.

SUBMITTER: Li N 

PROVIDER: S-EPMC5404495 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

altmetric image

Publications

A generic <sup>89</sup>Zr labeling method to quantify the in vivo pharmacokinetics of liposomal nanoparticles with positron emission tomography.

Li Nan N   Yu Zilin Z   Pham Truc Thuy TT   Blower Philip J PJ   Yan Ran R  

International journal of nanomedicine 20170420


Liposomal nanoparticles are versatile drug delivery vehicles that show great promise in cancer therapy. In an effort to quantitatively measure their in vivo pharmacokinetics, we developed a highly efficient <sup>89</sup>Zr liposome-labeling method based on a rapid ligand exchange reaction between the membrane-permeable <sup>89</sup>Zr(8-hydroxyquinolinate)<sub>4</sub> complex and the hydrophilic liposomal cavity-encapsulated deferoxamine (DFO). This novel <sup>89</sup>Zr-labeling strategy allowe  ...[more]

Similar Datasets

| S-EPMC7460274 | biostudies-literature
| S-EPMC8758695 | biostudies-literature
| S-EPMC5457332 | biostudies-literature
| S-EPMC4315484 | biostudies-literature
| S-EPMC7086351 | biostudies-literature
| S-EPMC6205629 | biostudies-literature
| S-EPMC8300002 | biostudies-literature
| S-EPMC8319402 | biostudies-literature
| S-EPMC10409919 | biostudies-literature
| S-EPMC5752591 | biostudies-literature