Ontology highlight
ABSTRACT:
SUBMITTER: Chae YK
PROVIDER: S-EPMC5423636 | biostudies-literature | 2017
REPOSITORIES: biostudies-literature
Chae Young Kee YK Kim Seol Hyun SH Markley John L JL
PloS one 20170509 5
Escherichia coli has been the most widely used host to produce large amounts of heterologous proteins. However, given an input plasmid DNA, E. coli may produce soluble protein, produce only inclusion bodies, or yield little or no protein at all. Many efforts have been made to surmount these problems, but most of them have involved time-consuming and labor-intensive trial-and-error. We hypothesized that different metabolomic fingerprints might be associated with different protein production outco ...[more]